Do I need to separate my pregnant dog and her new puppies from other dogs?

It can cause her to be territorial and possessive around the puppies. The best thing to do is when she goes in to labor is to keep her separate from the other dogs so she can focus and relax to give birth. After the puppies are born give her a few days without the other dogs going over there. After that you can let the other dogs, one at a time, sniff the puppies and see how they react

No, you don;t need to keep her separated during pregnancy. Just make sure she has a quiet place for when the time comes to whelp. The gestation time is 63-65 days on average. Here is some information on whelping: https://www.petcoach.co/article/whelping-giving-birth-in-dogs/ Congratulations!

You don't necessarily have to separate them, I'd only do it if you see any signs of aggression between them. If one of them does give birth to a litter, then I would give that dog her own room and her own space, and keep them separated while the puppies are little. I hope this helps!

Dogs can become more protective when they have puppies. Unfortunately there is not really a way to stop this aggression as it is instinctual and hormonal. A better option is to keep strangers and other unknown persons and pets away from the new mother. Make sure she feels safe and that her litter is kept in a low traffic and quiet area. The more the mother is stressed, the more likely she will be to show aggression. This aggression will generally decrease as the puppies get older. If her aggression is to the point where she is dangerous or extremely disruptive, you may consider removing her from your breeding program and having her spayed. Always be positive towards her and never frustrated or angry. This is normal behavior for a mother. Best of luck and please feel free to post any additional questions.

Unless your dog has a history of hurting her pups or you noticed that she is trying to hurt them, there should be no reason that you cannot leave her alone with her puppies. She is a mother, and mothers usually take good care of their babies.

Monitoring throughout the 8 week period until the puppies are weaned is recommended, yes, although it may be difficult while you are sleeping. Having the mom and pups in your room and setting an alarm to check on them a few times a night is reasonable. If you are able to get someone to watch them that's great. Otherwise minimize the amount of time they are left alone as much as possible, and try to get a dog sitter if you will be gone more than an hour. Breeding dogs is a lot of work, and it is important to keep track of the mom and pups' progress by checking on them frequently. Here is some more information on birthing puppies https://www.vin.com/veterinarypartner/default.aspx?pid=19239&id=4951546

Yes. You will need to remove the mother from the puppy. I also recommend to have the mother spayed or to not breed her in the future. Not all dogs make good parents. Attacking the puppies is not a good quality for a mother dog to have. I hope this is helpful and please feel free to post any additional questions.