Guaranteed Analysis

Guaranteed Analysis per 2 Soft Chews: Moisture (Max.) 21.5%, *alpha-Amylase (Aspergillus oryzae) 2,320 SKBU1, *Lipase (Rhizopus oryzae) 210 LU2, *Cellulase (Trichoderma longibrachiatum) 75 CMCU3, *Protease (Papaya) 22,100 PU4, *Bacillus subtilis (PB6 Probiotic) 1 Billion CFU, *Bacillus coagulans (Probiotic) 100 Million CFU (All values are minimum unless otherwise noted.). *Not recognized as an essential nutrient by the AAFCO Dog Food Nutrient Profiles. (1) One SKB unit is defined as the amount of enzyme needed to form 10 mg of reducing sugars from 1% soluble starch solution at pH 5.0 and 40 degreeC in 30 minutes. (2) One FCC Lipase Unit (LU) is defined as that quantity of enzyme that will liberate the equivalent of one umol of acid (H+) per minute from the substrate, under the conditions of the assay. The assay is based on a five-minute hydrolysis of an olive oil substrate at pH 6.5 and 30 degree C. The assay is based on a five-minute hydrolysis of an olive oil substrate at pH 6.5 and 30 degreeC. The fatty acids released on hydrolysis of the glycerol esters are determined by titration with sodium hydroxide. (3) One carboxymethyl cellulose unit (CMCU) is that amount of enzyme which liberates one micromole of reducing sugar (expressed as glucose equivalents) in one minute under the conditions of the assay. (4) The activity of papain is measured in the FCC PU; the assay of which is based on the hydrolysis of casein. This assay is based on a 60-minute proteolytic hydrolysis of a casein substrate at pH 6.0 and 40 degreeC. One PU (Papain Unit) is defined as that quantity of enzyme that liberates the equivalent of 1ug of tyrosine per hour under the conditions of the assay.